Michael Hooker Microscopy Facility (MHMF.ORG)

Acid Washing Coverslips

Aids adhesion of cells or polyamines/lamanin and gives a cleaner optical surface for DIC

• 1)  Heat cover slips in a loosely covered glass beaker in 1M HCl at 50-60oC for 4-16h.

• 2)  Cool to room temperature

• 3)  Rinse out 1M HCl with ddH2O

• 4)  Fill container with ddH2O and sonicate in water bath for 30 mins

• 5)  Repeat step 4

• 6)  Repeat step 4

• 7)  Fill container with 50% EtOH and 50% ddH2O and sonicate in water bath for 30 min

• 8)  Fill container with 70% EtOH and 30% ddH2O and sonicate in water bath for 30 min

• 9)  Fill container with 95% EtOH and sonicate in water bath for 30 min

• 10) Fill container with 95% EtOH

		Copyright 2001-10  Dr. M. Chua, Office of Research, School of Medicine, University of North Carolina, Chapel Hill, NC 27599
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