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Michael Hooker Microscopy Facility (MHMF.ORG)

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Perkin Elmer Yokogawa Nipkow spinning disk confocal

Operating the System

Powering up: - (if everything is off)


Optical setup

Setting up for transmitted light imaging
--> If transmitted illumination is not required then go to "for fluorescence only" below

Setting up for fluorescence light imaging

 

For imaging:


Acquiring Images Using SimplePCI with the Spinning-Disc Confocal

Shut down SimplePCI and log off when you are finished


Power down procedure:

  1. Laser off first (the following order is VITAL to the health of the laser!!)
    1. Pull standby switch forward
    2. Wait for ~60 seconds
    3. Turn power knob counterclockwise until it stops
    4. Turn key to Off (counter clockwise)
    5. The cooling fan will shut itself off ~10 minutes after the key is turned to the "Off" position.
  2. Exit the SimplePCI software
  3. Turn off the switch on the power strip next to the monitor.  This turns off the:
  4. Turn off the scan head by turning the key two clicks to the left
  5. Turn off the microscope incandescent light (left side, front button)
  6. Turn off the XBO (Fluorescence) lamp (always last!!)

 


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Copyright 2001-2015  Dr. M. Chua, School of Medicine, University of North Carolina, Chapel Hill, NC 27599
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Last Updated: 2014-07-24